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Apelin-13, [pGlu1, Ala13] (Human, Rat, Mouse, Bovine) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-057-30 100 µl $450.00

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Protocol for Immunohistochemistry: 
Tissue SampleHuman Kidney Tissue
Fixative10% Formalin
EmbeddingParaffin
Negative controlNo primary antibody
PretreatmentIntact
Blocking2% Normal Goat Serum
Primary AntibodyRabbit Anti-Apelin-13 [pGlu1, Ala13] (Human, Rat, Mouse, Bovine) Serum (Catalog No.: H-057-30)
Optimal Dilution1:800 (1 hour at RT)
Secondary AntibodyGoat anti-Rabbit IgG, Biotinylated (1:400)
Amplification Streptavidin-HRP (Vector), 1:400, 30 min
Detection systemHRP
SubstrateDAB (Sigma)
CounterstainedHematoxylin
Catalog #H-057-30
FamilyApelin
Standard Size100 µl
SequencepGlu - Arg - Pro - Arg - Leu - Ser - His - Lys - Gly - Pro - Met - Pro - Ala
SpeciesHuman, Mouse, Rat, Bovine
HostRabbit
ImmunogenApelin-13 [pGlu1, Ala13] (Human, Rat, Mouse, Bovine)
ReconstitutionFor best and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
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