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Catestatin

A New Hypertension / Stress / Insomnia / Cancer Biomarker

  • acts as a potent endogenous nicotinic cholinergic antagonist to inhibit catecholamine release.
  • induces release of histamine from mast cells.
  • displays potent antimicrobial activity

Human Catestatin RIA Kit (RK-053-27)

    

Rat(RK-053-28) and Mouse(RK-053-29) Catestatin RIA Kits

    

Human(EK-053-27) and Rat(EK-053-29) Catestatin EIA Kits

    

Human(FEK-053-27) and Rat(FEK-053-29) Catestatin Fluorescent EIA Kits

    

 

Mapping in Human Adrenal Medullary Tissue (H-053-27 / H-053-29)

    

Protocol for antibody staining

Tissue Sample

Human adrenal medullary

Fixative

10% formalin

Embedding

Paraffin

Negative Control

No primary antibody

Pretreatment

N/A

Blocking

3% H2O2, 2% Normal Goat Serum

Primary Antibody

Rabbit anti-Catestatin (Human) Antibody (Cat. No.: H-053-27)

Optimal Dilution

1: 500

Secondary Antibody

Goat Anti-Rabbit IgG, Biotinylated (1:400), 30 min

Amplification

Streptavidin-HRP (Vector), 1:400, 30 min

Detection System

HRP

Substrate

DAB (Sigma), 3 min

Counterstained

Hematoxylin, 30 sec


Tissue Sample

Human adrenal medullary tissues

Fixative

10% formalin

Embedding

Paraffin

Negative Control

No primary antibody

Pretreatment

N/A

Blocking

3% H2O2, 2% Normal Goat Serum

Primary Antibody

Rabbit Anti-Catestatin (Rat) Antibody (Cat. No.: H-053-29)

Optimal Dilution

1: 500

Secondary Antibody

Goat Anti-Rabbit IgG, Biotinylated (1:400), 30 min

Amplification

Streptavidin-HRP (Vector), 1:400, 30 min

Detection System

HRP

Substrate

DAB (Sigma), 3 min

Counterstained

Hematoxylin, 30 sec

 

Mapping in Human Pancreas Tissue (H-053-27 / H-053-28)

    

Protocol for antibody staining

Tissue Sample

Human pancreas tissues

Fixative

10% formalin

Embedding

Paraffin

Negative Control

No primary antibody

Pretreatment

N/A

Blocking

3% H2O2, 2% Normal Goat Serum

Primary Antibody

Rabbit Anti-Catestatin (Mouse) Antibody (Cat. No.: H-053-28)

Optimal Dilution

1: 500

Secondary Antibody

Goat Anti-Rabbit IgG, Biotinylated (1:400), 30 min

Amplification

Streptavidin-HRP (Vector), 1:400, 30 min

Detection System

HRP

Substrate

DAB (Sigma), 3 min

Counterstained

Hematoxylin, 30 sec


Tissue Sample

Human pancreas tissues

Fixative

10% formalin

Embedding

Paraffin

Negative Control

No primary antibody

Pretreatment

N/A

Blocking

3% H2O2, 2% Normal Goat Serum

Primary Antibody

Rabbit anti-Catestatin (Human) Antibody (Cat. No.: H-053-27)

Optimal Dilution

1: 500

Secondary Antibody

Goat Anti-Rabbit IgG, Biotinylated (1:400), 30 min

Amplification

Streptavidin-HRP (Vector), 1:400, 30 min

Detection System

HRP

Substrate

DAB (Sigma), 3 min

Counterstained

Hematoxylin, 30 sec

 

Western Blot Analysis of Chromogranin A in Rat Kidney Tissue (H-053-27)

2

 

Related Articles

Chromogranin A: a surprising link between granule biogenesis and hypertension

cgA

Relationship of CHGA-mediated dense-core secretory granule (DCG) biogenesis, catecholamine (CA) secretion, and its subsequent inhibition by the CHGA-derived peptide catestatin in the maintenance of blood pressure by the adrenal gland. CHGA, as a full-length molecule, initiates dense-core secretory granule biogenesis at the trans-Golgi network of adrenal chromaffin cells. Current data suggests that CHGA enhances granule biogenesis by preventing posttranslational degradation of other granule proteins in the Golgi complex. In the cytoplasm, catecholamine is synthesized and transported into the dense-core secretory granules via vesicular monoamine transporters. Upon stimulation by acetylcholine (Ach), catecholamine is coreleased with CHGA and catestatin from the granules. Secreted catecholamine triggers cardiovascular target cells to augment blood flow. This sympathoadrenal activity is then antagonized by the action of catestatin on cholinergic receptors to inhibit catecholamine secretion. [Ca2+]i, intracellular calcium concentration.
Kim et al. J. Clin. Invest. 115:1711-1713 (2005)

 

%053-27%;%053-28%;%053-29%


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