Phoenix Pharmaceuticals has successfully applied antibodies onto
magnetic particles. Magnetic particles are a convenient affinity support for
a variety of assays, which allow easy purification of the target without
columns or centrifugation. PhoenixParticles are available pre-coated with
Protein A, Protein G, streptavidin, anti-mouse or anti-rabbit antibodies.
Analysis of plasma or
small body fluid samples for MODI-TOF MS. This magnetic-bead-based platform amenable to high-throughput peptide
capture and demonstrated that antibody capture followed by mass spectrometry
can achieve ion signal enhancements on the order of 103, with
precision (CVs <10%) and accuracy.
respond well to magnetic fields but have no magnetic memory.
separations require a short 2- to 10-minute bench-top procedure.
specifications – surface area: >100 m2/g, 1-2 µm or 0.6 –1 µm diameter depending on
are available pre-coated with Streptavidin, Protein A or G, antibodies and can be used for either
positive or negative selection .
can be isolated by binding directly to primary antibody or PhoenixParticles.
type isolated by PhoenixParticles is determined by the primary antibody bound
indirectly to the secondary antibody coated bead.
and simple method for peptide/protein purification or removal of un-needed
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