Tissue Sample

Rat Lung

Fixative

10% formalin

Embedding

Paraffin

Negative Control

No primary antibody

Pretreatment

Target Retrieval 10 min (micro-wave oven)

Blocking

2% Normal Goat Serum

Primary Antibody

Rabbit Anti-APJ (357-377) (Rat) Antiserum (Catalog No.:H-001-79)

Optimal Dilution

1:1000 (1 hour at RT)

Secondary Antibody

Goat Anti-Rabbit IgG, Biotinylated (1:400), 30 min

Amplification

ABC (Vector) (1:400, 30 min)

Detection System

HRP

Substrate

DAB (Sigma), 3 min

Counterstained

Hematoxylin, 30 sec