Apelin Receptor APJ Antibody

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Apelin Receptor APJ Antibody

Mapping in Rat Lung Tissue (H-001-79)

APJ APJ

APJ(357-377) (Rat) Protocol for Immunohistochemistry

Tissue Sample	Rat Lung
Fixative	10% formalin
Embedding	Paraffin
Negative Control	No primary antibody
Pretreatment	Target Retrieval 10 min (micro-wave oven)
Blocking	2% Normal Goat Serum
Primary Antibody	Rabbit Anti-APJ (357-377) (Rat) Antiserum (Catalog No.:H-001-79)
Optimal Dilution	1:1000 (1 hour at RT)
Secondary Antibody	Goat Anti-Rabbit IgG, Biotinylated (1:400), 30 min
Amplification	ABC (Vector) (1:400, 30 min)
Detection System	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 sec

Western blot analysis of Apelin Receptor APJ in Rat Lung Tissue (G-001-79)

apelin receptor

Apelin

%001-79%

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