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GPCR Peptide Ligand Library--A Reverse Pharmacology Approach
The library is continuously updated to include all new and current peptides, receptors and references.
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Phoenix's Peptide Receptor Ligand Libraries, a broad set of peptides (thousands),
enables a "reverse pharmacology" approach to identify unknown
membrane receptors, G-protein-coupled receptors, ion channels, intracellular
signaling enzymes and receptors.
Feighner et al. (1), successfully
identified a human motilin receptor using the Phoenix Gastrointestinal Peptide
Library and HEK293 cells in which a human motilin receptor was coexpressed
with aequorin, a bioluminescent calcium sensitive reporter protein. A similar
approach was taken by Chambers et al. (2), to identify an orphan G-protein
coupled receptor as a human MCH receptor, using over 500 naturally occurring
and putative neuropeptides.
Recently, neuromedin U has been identified as a natural ligand for two orphan G protein coupled receptors, FM-3 and FM-4 (3)
References:
1. Feighner, S.D. et al., Science 284, 2184-2188 (1999)
2. Chambers, J. et al., Nature 400, 261-265 (1999)
3. HOWARD, A.D. et al.Identification of receptors for neuromedin U
and its role in feeding. Nature 406, 70-75 (July 6, 2000).