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PHOENIX PHARMACEUTICALS, INC.
The Peptide Elite |
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| Relaxin-3 Receptor-2/GPCR 142 Immunohistochemistry
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Relaxin-3 Receptor-2 /GPCR 142 Immunohistocheistry Protocol
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Tissue
Sample |
rat hypothalamus
tissue |
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Fixative |
10% Formalin |
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Embedding |
Paraffin |
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Negative
control |
No primary
antibody |
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Pretreatment |
Intact |
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Blocking |
2% Normal
Goat Serum |
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Primary
Antibody |
Rabbit
Anti-Relaxin-3 Receptor-2/GPCR 142 (310-330) (Human) Serum
(Catalog No.:H-001-86) |
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Optimal
Dilution |
1:50~100
(1hour at RT) |
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Secondary
Antibody |
Goat anti-Rabbit
IgG, Biotinylated (1:400, 30 min) |
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Amplification |
ABC (Vector)
(1:400, 30 min) |
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Detection
system |
HRP |
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Substrate |
DAB (Sigma),
3 min |
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