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PHOENIX PHARMACEUTICALS, INC.
The Peptide Elite |
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| Ghrelin Immunohistochemistry |
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Ghrelin Immunohistochemistry
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Tissue
Sample |
Rat Stomach |
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Fixative |
10% Formalin |
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Embedding |
Paraffin |
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Negative
control |
No primary
antibody |
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Pretreatment |
Target
retrieval 25 min (Steam) |
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Blocking |
2% Normal
Goat Serum |
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Primary
Antibody |
Rabbit
Anti-Ghrelin (Human) Antiserum (Catalog No.:H-031-30 ) |
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Optimal
Dilution |
1:400
(1hour at RT) |
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Secondary
Antibody |
Goat anti-Rabbit
IgG, Biotinylated (1:400, 30 min) |
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Amplification |
ABC (Vector)
(1:400, 30 min) |
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Detection
system |
HRP |
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Substrate |
DAB (Sigma),
3min |
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Counterstained |
Hematoxylin,
30 Sec |
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