|
|
 |
PHOENIX PHARMACEUTICALS, INC.
The Peptide Elite |
|
|
|
| Angiotensin II Immunohistochemistry |
 |
Angiotensin II Immunohistochemistry
|
 |
| |
 |
Tissue
Sample |
Rat
kidney |
 |
Fixative |
10% Formalin |
|
Embedding |
Paraffin |
|
Negative
control |
No primary
antibody |
|
Pretreatment |
Pronase |
|
Blocking |
3% H2O2,
2% Normal
Goat Serum |
|
Primary
Antibody |
Rabbit
Anti-Angiotensin II Antiserum (Catalog No.:H-002-12) |
|
Optimal
Dilution |
1:2000
(1hour at RT) |
|
Secondary
Antibody |
Goat anti-Rabbit
IgG, Biotinylated (1:400, 30 min) |
|
Amplification |
ABC (Vector)
(1:400, 30 min) |
|
Detection
system |
HRP |
|
Substrate |
DAB (Sigma),
3 min |
|
Counterstained |
Hematoxylin,
30 Sec |
|
|
|
 |
|
 |
|
